Reporter

Part:BBa_K4799609

Designed by: Wanjun Chen   Group: iGEM23_BGI-MammothEdu-China   (2023-10-10)


pAtMT1a_GUS_T-Nos

pAtMT1a is a promoter. It is added before the reporter gene β-Glucuronidase (gus) and NOS terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 25
    Illegal SpeI site found at 1374
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 25
    Illegal NheI site found at 3183
    Illegal SpeI site found at 1374
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 25
    Illegal BglII site found at 1165
    Illegal BamHI site found at 1148
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 25
    Illegal SpeI site found at 1374
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 25
    Illegal SpeI site found at 1374
    Illegal NgoMIV site found at 1598
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1398
    Illegal BsaI.rc site found at 2013
    Illegal SapI.rc site found at 503
    Illegal SapI.rc site found at 2184


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Categories
Parameters
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